Figure 4.

Effect of Sox2 overexpression on endogenous Sox2:Oct-3/4 target gene expression. (A) F9 EC cells were transfected with either GFP or GFP-Sox2, and GFP positive cells were collected and processed as described in the Materials and methods section. Between these two cell populations, the levels of expression of five genes were compared after normalizing the values to the expression of GAPDH. Target gene expression in GFP-Sox2-transfected cells is reported as a fraction of the corresponding expression in control cells (GFP-transfected). The values shown represent the mean and standard deviation after averaging values from two separate isolates of GFP positive cells and at least two qPCR assays of each gene. (B) Western blot analysis of Sox2 protein in F9 EC cells transfected with the GFP-Sox2 expression plasmid and collected by flow cytometry. GFP-Sox2 and endogenous Sox2 are indicated.