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. 2007 Feb 25;35(6):1773–1786. doi: 10.1093/nar/gkm059

Figure 6.

Figure 6.

Effect of Sox2 overexpression on the HSV TK promoter. Schematics of each of the promoter/reporter constructs are shown. The gray box indicates the FGF-4 enhancer (HMG/POU cassette is indicated with an asterisk) and the basal TK promoter is represented by a black box adjacent to the CAT reporter gene. F9 EC cells were transfected with 12 µg of the indicated promoter/reporter gene construct plus 1 µg of the CMV-β-gal expression vector. Where indicated, 3 µg of the Sox2 expression vector was co-transfected. Reporter activity was normalized to that of β-galactosidase and the activity of the TK promoter/reporter construct (with no enhancer and no overexpression) was set to 1. All other values are shown relative to that particular gene construct (TK). The data shown represents the mean and standard deviation of duplicate samples from a representative experiment. This experiment was repeated twice and similar results were obtained.