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. 2007 Feb 25;35(6):1773–1786. doi: 10.1093/nar/gkm059

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Effect of Oct-3/4 and Nanog overexpression on promoter activity. (A) Effect of Oct-3/4 overexpression on Oct-3/4 promoter activity. (B) Effect of Nanog overexpression on Nanog and Sox2 promoter activity. Schematics of each of the promoter/reporter constructs are shown. The gray boxes indicate critical enhancers, the basal promoter is represented by a black box adjacent to the CAT reporter gene, and the relative HMG/POU cassette is indicated with an asterisk. F9 EC cells were transfected as indicated with 12 µg of either the Oct-3/4, Nanog or Sox2 promoter/reporter gene constructs (Oct-3/4 + E, Nanog + E or Sox2 + US/DSE) plus 1 µg of the CMV-β-gal expression vector. Where indicated, 1 or 3 µg of the Oct-3/4, Sox2 or Nanog expression vector was co-transfected. Reporter activity was normalized to that of β-galactosidase and the activity of each individual promoter/reporter construct (with no overexpression) was set to 1. Overexpression values are shown relative to each particular gene construct. The data shown represents the mean and standard deviation of duplicate samples from a representative experiment. Each experiment was repeated twice and similar results were obtained.