Table 2.

Oligonucleotide primers used for primer extension analysis

Gene	Nucleotide sequences (5′ → 3′)	Complementary regionc
hilA	TAATAATATTGTTATAACTAACTGTGATTA	∼−216 to −245
invFA	CATTGTGTCGGCTTTCAGAAAATGACATAT	∼−1 to +28
invFD	GGAGTTAATATGAAAAAATTTTATAGCTGT	∼−447 to −476
hilC	GGAAATTTGTTCGGCTGTTGAAGGTGATTA	∼+45 to +74
hilD	TTTAATTTGCTGCCGGGTATTTGTCAAAAG	∼+73 to +102
hilE	CAATGAAAGAACGTTCCATTTTCCAGCCA	∼+2 to +30a
hilE	ATATCAATATCATTTCTTATTTTTATCCGA	∼−188 to −217b
prgH	CTGTCAGCAATGGAAACTCACAGCCGTTCA	∼+71 to +100
sigD	AGGTTTTTTGTAGGCTTTTAAAAGCCTCCT	∼+44 to +73

^ahilE3 primer used for the analysis of P1 and P2 transcript.

^bhilE5 primer used for the analysis of P3 transcript.

^cNumbers of nucleotides were determined relative to translational start site respectively.