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. 2007 Mar 1;35(6):e46. doi: 10.1093/nar/gkm086

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — pMU1, an integrating lux reporter plasmid. tfd, the major transcriptional terminator of phage fd; to, transcriptional terminator of phage λ; ori, the origin of replication from pUC18; aac3(IV) apramycin-resistance cassette selectable in E. coli and Streptomyces; oriT, origin of transfer from the RK2 plasmid; int and attP, the integrase gene and attachment site of the ΦBT1 phage, respectively. The multiple cloning site consists of EcoRV, BamHI and KpnI site (non-unique sites are marked with*). The multiple cloning site is followed by translational stop codons in all three reading frames (S1, S2, S3) and a ribosome binding site (RBS).