Table 2.
Plasmids used in this work
| Plasmid | Description | Reference or source |
|---|---|---|
| Streptomyces coelicolor A3(2) | ||
| pRT801 | Integrative vector for Streptomyces; oriT(RK2) int attP (ΦBT1) aac(3)IV | (30) |
| pMU1 | Promoterless luxCDABE operon, flanked by transcriptional terminators, preceded by in-frame stop codon and ribosome binding site, aac(3)IV | This work |
| phrdBlux | hrdB promoter transcriptional fused to luxCDABE | This work |
| pramClux | ramC promoter transcriptionally fused to luxCDABE | This work |
| pwhiEp1lux | whiEp1 promoter transcriptionally fused to luxCDABE | This work |
| Escherichia coli | ||
| pBluescript II SK+ | pUC18 derivative for cloning in E. coli, bla | Stratagene |
| PBluescript + luxCDABE | Assembled luxCDABE operon | This work |
| pDONR221 | Gateway cloning vector, kan | Invitrogen |
| pDONR221 + luxA22 | luxA allele | This work |
| pDONR221 + luxB23 | luxB allele | This work |
| pDONR221 + luxC4 | luxC allele | This work |
| pDONR221 + luxD2 | luxD allele | This work |
| pDONR221 + luxE5 | luxE allele | This work |