Figure 1.

Sequence alignment between R.PabI and its homologs identified in protein sequence databases. jhp0455 and HPAG1_0479 are putative translation products of open reading frames (ORFs) from Helicobacter pylori strains J99 and HPAG1. HP0504 + 0505 and Hac_0824 + 0825 are reconstructed translation products of pseudogenes disrupted by frameshift mutations in H. pylori 26695 and H. acinonychis str. Sheeba. CUP0001 is a product of an incomplete ORF from Campylobacter upsaliensis RM3195. Amino acid residues with similar physico-chemical properties are indicated by color (hydrophobic—gray, negative—red, positive—blue, polar—green, cysteine—brown). Identical residues are highlighted. Residues analyzed by alanine mutagenesis in this work are indicated in the top by ‘-’ (mutant is partially active) or ‘=’ (mutant's activity is undetectable). The bottom panel shows secondary structure determined for R.PabI.