Figure 5.

Kissing dimers (KDs) of all six SL1 constructs were incubated at ambient temperature for 2 h (left panels) or 18 h (right panels) with NCp7 in the RNA strand-to-protein ratios of 2:0, 2:2, 2:4 and 2:8. RNA was ethanol-precipitated after phenol–chloroform extraction, dialyzed in the 1× dimerization buffer and separated in native PAGE in the TBE buffer. ‘LD’ indicates the position of the dimer bands, which correspond to the linear dimers at these conditions. KDs dissociate to monomers during PAGE (shown by ‘M’). Panels (A–F) show results for SL1-wt, SL1-yb, SL1-yf, SL1-yy, SL1-nb and SL1-es, respectively.