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. 2007 Mar 27;35(7):2295–2301. doi: 10.1093/nar/gkm104

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Location of the m²G966, m²G1207 residues of the 16S rRNA and m²G1835, m²G2445 residues of the 23S rRNA in the ribosomal structure and their interactions. (A) Position of the 16S rRNA residue m²G966 relative to the P-site-bound tRNA (17). The 30S subunit is shown in yellow. P-site-bound tRNA is shown in green. Helix 31 is indicated as a gray tubing. Modified nucleotides m²G966 and m²G967 are shown as a blue and red Van-der-Vaals spheres accordingly and labeled. (B) Closer view of the position of the 16S rRNA residue m²G966 relative to the P-site-bound tRNA. 16S rRNA helix 31 is shown on the left, P-site-bound tRNA anticodon is shown on the right. (C) Position of the 16S rRNA residue m²G1207 relative to the A-site-bound tRNA anticodon (23). The 30S subunit is shown in yellow. A-site-bound tRNA anticodon is shown in green. Helix 34 is indicated as a gray tubing. Modified nucleotide m²G1207 is shown as red Van-der-Vaals spheres and labeled. Nucleotide C1054 making a direct contact with A-site tRNA anticodon is shown as wireframe and labeled. (D) Closer view of the position of the 16S rRNA residue m²G1207 relative to the A-site-bound tRNA. 16S rRNA helix 34 is shown on the left, A-site-bound tRNA anticodon is shown on the right. (E) Position of the 23S rRNA residue m²G1835 in the E. coli ribosome (27). Position of the m²G1835 residue relative to the ribosomal subunits. The 30S subunit is shown in yellow; the 50S subunit is shown in blue. Modified nucleotide m²G1835 is shown as red Van-der-Vaals spheres and labeled. (F) Details of structural environment of the m²G1835 in the ribosome. Position of the methyl group is marked by blue circle. Surrounding nucleotides are shown as wireframe and labeled. 16S rRNA nucleotides are on the left side, 23S rRNA nucleotides are on the right side. Border between the subunits is indicated by line. (G) Position of the 23S rRNA residue m²G2445 in the E. coli 50S subunit (27). Position of the m²G2445 residue relative to the large ribosomal subunit, shown in blue and labeled. The orientation of the 50S subunit is as viewed from the 30S subunit. (H) Details of structural environment of the m²G2445 in the 50S subunit. Position of the methyl group is marked by blue circle. Surrounding nucleotides are shown as wireframe and labeled.