Skip to main content
. 2007 Mar 28;35(7):2428–2439. doi: 10.1093/nar/gkm175

Figure 1.

Figure 1.

Ccr4-Not complex components genetically interact with BUR1 and BUR2 and play a role in transcription elongation. (A) Tetrad analysis of bur2Δ (KMY201) and BY4741 Ccr4-Not deletion or not4Δ (KMY40) BY4741bur2Δ diploids. Circles indicate double knock-out strains. Relevant genotypes of the haploid strains obtained are indicated (B) Plasmid-shuffle analysis of bur1-23 and not4Δ double-mutant strains containing pRS316-BUR1. Here, 10-fold serial dilutions of the indicated strains were spotted on SC and SC containing 0.1% 5FOA. (C) 6-Azauracil sensitivity assay. Here, 10-fold serial dilutions of the indicated strains were spotted on SC-U and SC-U containing 6-AU (100 μg/ml). (D) In vivo transcription elongation analysis of a long GC-rich reporter. BY4741 and not4Δ (KMY58) strains transformed with a GAL1pr-LacZ plasmid (pGR422) were grown in a medium containing raffinose and shifted to a medium containing galactose for 0, 45 and 90 min. Northern blot analysis on LacZ and endogenous GAL1 mRNA was performed using 18S rRNA as a control.