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. 2007 Mar 13;35(7):2153–2166. doi: 10.1093/nar/gkm068

Figure 3.

Figure 3.

EMSAs of 32P-labeled DNA fragments (probes) containing predicted Fur boxes and promoters of (A) gloA and feoP, (B) copB and abcS4, (C) iscR and phoB, (D) hppH and (E) fdx1 in the presence of FurAF (Fur), FurAF plus anti-Fur serum (α-Fur) or cold probe (P*) as indicated. (F) EMSA of a 32P-labeled probe containing no predicted Fur box and corresponding to a low G + C DNA fragment of pUC18 vector. 1 = probe DNA, (A–D) 2 = shift with 300 nM FurAF, 3 = supershift with anti-Fur antibody; (E) 2 = shift with 300 nM FurAF, 3 = shift with 400 nM FurAF, 4 = supershift, 5 = cold probe competition, 6 = absence of shift in the presence of the anti-Fur antibody alone. (F) absence of shift in the presence of: 2 = 100 nM FurAF, 3 = 200 nM FurAF, 4 = 300 nM FurAF, 5 = 400 nM FurAF and occurrence of shift in the presence of 6 = 500 nM FurAF.