Skip to main content
. 2002 Oct 15;16(20):2627–2632. doi: 10.1101/gad.239102

Figure 1.

Figure 1

dS6K is constitutively activated in dTsc- but not dPTEN-depleted Drosophila Kc167 cells. (A) Real-time PCR analysis following dTsc1 and dPTEN dsRNAi. (B) Analysis of dS6K activity following dTsc1 dsRNAi. Co, untreated control; RNAi, dsRNAi targeting dTsc1; +ins, insulin (100 nM) stimulation for 30 min; RAD, 15 min RAD001 (20 nM) pretreatment. (Top panel) In vitro dS6K activity. (Bottom panels) Western blot analysis of dS6K T398 phosphorylation (top) and dS6K band-shift (bottom). (C) Analysis of dPKB activity following dTsc1 dsRNAi treatment. (Top panel) In vitro dPKB activity assay. CT, Crosstide. (Bottom panels) Western blot analysis of dPKB S505 phosphorylation (top) and total dPKB (bottom). (D) Analysis of atypical dPKC activity following dTsc1 dsRNAi treatment. (Top) In vitro dPKC activity assay using myelin basic protein as substrate (MBP). (Bottom) Western blot of total atypical dPKC. (E) Analysis of dPKB activity following dPTEN dsRNAi treatment. Panels are as in C. (F) Analysis of dS6K activity following dPTEN dsRNAi treatment. The top panel is as in B, the bottom panels are Western blot analyses of dS6K T398 phosphorylation (top) and total dS6K (bottom).