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DNA protection assays against DNase I cleavage. Reaction components and pH conditions are indicated on top of each lane. In each lane linearized plasmid DNA was 20 nM. When present DNase I was 0.3 U and HP-NAP was 2 µM. DNase I treatment was carried out for one minute. All reaction mixtures contained 3 mM MgCl2. Agarose gel was stained with SYBR Green I.
