Figure 2.

Comparison of DARPP-32 phosphorylation in response to a D1 agonist and forskolin in WT and HD mice. Striatal slices from 6-week-old mice were treated with either a dopamine D1 receptor agonist (a), SKF81297 (SKF), or an activator of adenylyl cyclase (b), forskolin (FSK). Homogenates of the slices were subjected to SDS/PAGE, proteins were transferred to polyvinylidene difluoride membranes, and phospho-Thr³⁴ DARPP-32 was analyzed by immunoblotting using a phosphorylation state-specific antibody. Blots were reprobed to determine total levels of DARPP-32 (Lower).