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. 2002 Nov 1;16(21):2772–2777. doi: 10.1101/gad.1021002

Figure 1.

Figure 1

SNAP190 (884–910) assists Oct-1 POU DNA binding to the U1 octamer element. (A) The DNA sequences used in the gel shifts shown below. The octamer sequence is numbered 1–8, with the equivalent base on the opposite strand indicated by a prime. The two base pair changes in the U1 octamer relative to the H2B octamer sequence are shown in bold. (Inset) Complete U1 octamer oligonucleotide sequence used in crystallization. (B) Electrophoretic mobility shift assays were performed using 0.1 ng (lanes 2,5) or 1 ng (lanes 3,6) of human Oct-1 POU-domain protein with DNA probes containing a human histone H2B (lanes 1–3) or U1 snRNA (lanes 4–6) octamer element. Lanes 1 and 4 contain the probes alone. The position of the POU complex is indicated (DNA/Oct-1). (C) Electrophoretic mobility shift assays were performed using DNA probes containing a human histone H2B (lanes 1,2) or U1 snRNA octamer element (lanes 3–8) with 1 ng (lane 2) or 30 ng (lanes 4–6) of human Oct-1 POU-domain protein alone (lanes 2,4), with 10 μg of SNAP190 peptide (lane 5), or with an equimolar amount of a control peptide (lane 6). Lanes 7 and 8 contain the SNAP190 or control peptides alone, respectively. Lanes 1 and 3 contain probe DNA alone. The position of the POU complex is indicated (DNA/Oct-1).