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. 2002 Nov 1;16(21):2778–2791. doi: 10.1101/gad.1030602

Figure 6.

Figure 6

A model for EJC assembly. EJC assembly is initiated as early as the H complex, when REF and SRp20 become associated with the 5′ exon. Additional EJC components join the complex prior to exon ligation. As the splicing reaction proceeds through exon ligation and mRNP release, at least three additional proteins become closely associated with the RNA, while other factors may remain with the spliceosome. Association of some EJC proteins becomes less stable after mRNP release. Proteins identified by cross-linking are named according to their apparent molecular weights, whereas p32/REF and p25/SRp20 were identified by denaturing immunoprecipitation. Proteins lacking a molecular-mass designation (bottom section) were shown to be present in the C complex or spliced mRNP by mass spectrometry and/or Western analysis.