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. 2002 Dec 1;16(23):3074–3086. doi: 10.1101/gad.242602

Figure 4.

Figure 4

Heparan sulfation of CD44 is not required for Met signaling. (A) HGF-induced Erk phosphorylation was measured in HT29, ASv4–7Δ15, and ASv1–10 cells. Where indicated, cells were pretreated with heparinase II (Hase) at 37°C for 3 h prior to HGF addition. (B) Western blot analyses using a ΔHS antibody are shown in the right panels. Where indicated, cells had been pretreated with heparinase II (6U/mL) at 37°C for 3 h. The lysates were probed by Western blotting using Hermes 3 (for HT29 cells) or 5G8 (for AS transfectants) antibodies.