Figure 2.

RNase protection assay for the detection of ret, GDNF, NTN, and gfrα receptors during brain development. The brain was collected at different time points before or after birth. Total RNAs were extracted and submitted to RNase protection assay by using the indicated probes. Autoradiograms are presented at Left. (Right) The graphs represent the average of radioactivity value detected on a PhosphorImager expressed in arbitrary units and displayed as [(value X/value GAPDH) × 1,000]. To be able to show the grfα3 regulation in the same graph as the two other members of the family, values for gfrα3 was multiplied by 40. P, postnatal day; Adt, adult.