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. 2002 Dec 1;16(23):3087–3099. doi: 10.1101/gad.248902

Figure 1.

Figure 1

The p27 5′UTR. (A) Structure of the largest p27 5′UTR. The largest human p27 5′UTR is aligned to the 5′ flanking region of the mouse p27 genomic sequence (accession nos. U77915 and U10440). The upstream ORF (uORF) is marked with a line. (B) p27 mRNA contains an extended 5′UTR that is produced independently from cell cycle position. RNAse protection of 14 μg RNA isolated from proliferating (lane 4) or arrested HeLa cells (lane 5, lovastatin; lane 6, thymidin). Total RNA or 14 μg yeast tRNA hybridized to a 681-nucleotide antisense fragment containing the p27 5′UTR from nucleotides −3 to −575. Protected RNAs were detected by autoradiography. The major protection products are indicated by a clamp; additional protection products are indicated by arrows. (Lane 1) RNA marker; fragment length in nucleotides is indicated. (Lanes 2,3) Yeast tRNA (14 μg) with (lane 3) or without (lane 2) RNase treatment. (Lanes 46) RNA from proliferating (lane 4), lovastatin-arrested (lane 5), or thymidine-arrested (lane 6) HeLa cells hybridized and treated with RNase.

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