Figure 4.

Cohesin release is not a prerequisite of chromosome compaction. Interphase nuclei were assembled from sperm chromatin in interphase LSS that had been mock-depleted, Plx1-depleted, aurora B-depleted, or Plx1/aurora B-depleted. After 2 h, a half volume of the corresponding CSF extract was added and the mixture was incubated for another 2 h. Samples were fixed and stained with DAPI (red; top row) and anti-XSA1 antibody (green; third row). Merged images are shown in the fourth row. Chromosomal regions were selected from the DAPI images (indicated by rectangles) and their close-ups are shown in the second row (indicated by an asterisk). All images of the antibody staining were taken using the same exposure time. Bar, 10 μm.