Table 6.
daf-4 acts cell-autonomously to regulate chemoreceptor gene expression and non–cell-autonomously to regulate dauer formation
| Strain
|
% animals expressinga
|
% dauers formedb
|
|
|---|---|---|---|
|
sra-6::gfp
|
str-2::gfp
|
||
| WT | 100 | 0 | 0 |
| daf-4(m63) | 4c | 100 | 100 |
| daf-4(m63); Ex[odr-4::daf-4] | 93d | 23 | 76 |
| daf-4(m63); Ex[osm-10::daf-4] | 91e | — | 85 |
| daf-4(m63); Ex[myo-3::daf-4] | 3 | — | — |
| daf-4(m63); Ex[osm-10::daf-4] | 81 | — | — |
| ASI killed (adults)f | |||
| daf-4(m63); Ex[osm-10::daf-4] mock ablated (adults)f | 100 | — | — |
All strains contain the pRF4 coinjection marker with the exception of ASI-killed and mock-ablated animals which contain the unc-122::gfp coinjection marker (Miyabayashi et al. 1999). Data shown are from two independent lines, each of which showed similar levels of rescue. n > 95 for each.
— indicates not done.
Expression was scored as sra-6::gfp expression in one to four neurons in the head. In ASI-killed animals, expression was scored as expression in both ASH neurons. Expression was scored as weak str-2::gfp expression in one or two ASI neurons.
Dauer formation was examined at 25.5°C as described previously (Lanjuin and Sengupta 2002).
3.1% of animals expressed GFP in one cell, and 1% expressed it in two cells.
2% expressed GFP in one cell, 32% expressed GFP in two cells, 14% expressed GFP in three cells, and 45% expressed GFP in four cells.
10% expressed GFP in one cell, 52% expressed GFP in two cells, 12% expressed GFP in three cells, and 17% expressed GFP in four cells. In animals in which GFP was expressed in two cells, both cells were identified as ASH by dye-filling (100%, n = 31).
Data shown for ASI-killed animals are from one line; n = 11 for ASI-killed animals; n = 22 for mock ablated animals. ASI neurons were killed in young adult animals, and GFP expression was scored after 4 d at 25°C.