Table 1.
Rescue of tooth development in explanted E13/E14 Lef1−/− molar rudiments by recombinant signal factors or heterologous tissues
|
|
Teeth/explants
|
|---|---|
| Signaling molecules | |
| BMP4 | 0/8 |
| SHH | 0/16 |
| FGF4 | 58/59 |
| FGF9 | 10/11 |
| FGF8b | 16/17 |
| FGF7 | 22/25 |
| FGF10 | 11/14 |
| Heterologous tissues | |
| Notochord | 0/27 |
| Limb ectoderm (AER) | 10/12 |
Explants were associated either with beads soaked in recombinant signal factors or with heterologous tissues. After 2 or 3 days in culture, the explants were transplanted under the kidney capsule to allow for full development (see text for details). AER, apical ectodermal ridge.