Table 3.
PVA-Specific Oligonucleotides Used in the Genomic Segment Amplification
| Primer pair (forward-reverse) | Region amplified (first and last nt) | Oligonucleotide sequence | |
|---|---|---|---|
| Forward | Reverse | ||
| 61475-FO15R | 2-1259 | aataaacaactacaaaaca | agtgtcctcatccatgttcaaattc |
| 5960-5961 | 1053-2426 | tattcaacaggggatgttttctggc | tccaaccctgtagtgcttcat |
| 51568-51567 | 2268-3317 | gaattttggttgatcacgatctca | cttacagtactccaattccaggc |
| FO14F-FO14R | 3099-4405 | caggctgtgaatggattgcaag | acttcattataacttgccacgtaca |
| 71832-6695 | 4227-5528 | gtgtccgccacaccaccagg | ctggaactgcactgcctctaagta |
| 71106-71105 | 5152-6375 | ttgagaaattttaaatgtgatgc | tcaagggcccaaaacctaatccaa |
| 72270-6281 | 6256-6983 | caacatctatgtttacaccc | ttgggtgtatactgcctctc |
| FO2F-FO1R | 6799-7570 | ttcccaaaggattcacagaaac | gtttcaattggtgcagctgt |
| FO1F-FO5R | 7180-8260 | gagaggcttacaacacaggatc | tcatgtgatctatcccactcc |
| FO5F-FO7R | 7869-9111 | caccagatggcacagttgtta | ctctcaaggtgcgttgaagacc |
| FO7F-4141 | 8747-9565 | gctatcttacaaaccaaagcaagt | ccctgacagttgaaacataaa |
Genome region coordinates follow the PVA-B11 sequence (AJ296311). Oligonucleotides are shown in the 5′ to 3′ direction.