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Overview of the deletion procedure. A, B, and C represent arbitrarily chosen DNA segments (homology boxes). Numbers refer to the length of oligonucleotides in basepairs. Polymerase chain reaction primers are labeled by lower case letters in bold (a, b, c, d, e, ab). S indicates an I-SceI cleavage site. Step-by-step description of the procedure can be found in the article.
