Table 2.
EST Assembly Results
| PHRAPa | CAP3b | CAP3c | |
|---|---|---|---|
| Total sequences analyzed | 14642 | 14642 | 15311 |
| Number of ESTs in contigs | 8464 | 8357 | 9481 |
| Number of contigs | 3119 | 2910 | 3138 |
| Number of singlets | 6178 | 6285 | 5830 |
| Number of putative transcripts (assembled sequences) | 9297 | 9196 | 8966 |
| Number of contigs containing: | |||
| 2–4 ESTs | N/A | 2626 | 2762 |
| 5–10 ESTs | N/A | 255 | 334 |
| 11–20 ESTs | N/A | 28 | 33 |
| 21–40 ESTs | N/A | 2 | 6 |
| >40 ESTs | N/A | 1 | 1 |
a
Default settings
b
High-quality ESTs assembled using high-quality, vector-trimmed sequence only. Default settings were used except minimum overlap was 40 bp and 95% identity (default is 30 bp, 75% identity).
c
High-quality ESTs assembled using high- and low-quality, vector-trimmed sequence, 3′ and reductant 5′ ESTs were included (these were treated as independent clone sequences to avoid error resulting from manual clone picking). Default settings were used except minimum overlap was 40 bp. These assembly results were used for all analyses in this study, except where noted.
EST, expressed sequence tag.