Table 7.
Signal analysis of an example microarray
| Number of spots | 635 nm | 532 nm | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Feature | Background | Feature | Background | ||||||
| Avg. | SD | Avg. | SD | Avg. | SD | Avg. | SD | ||
| Apis cDNAs | 7329 | 3.42 | 0.47 | 2.23 | 0.05 | 3.43 | 0.48 | 2.15 | 0.04 |
| Exogenous control 1 | 16 | 2.40 | 0.07 | 2.24 | 0.05 | 2.35 | 0.07 | 2.15 | 0.04 |
| Exogenous control 2 | 16 | 2.43 | 0.07 | 2.23 | 0.04 | 2.41 | 0.06 | 2.15 | 0.04 |
| Exogenous control 3 | 16 | 2.32 | 0.08 | 2.23 | 0.05 | 2.26 | 0.08 | 2.15 | 0.04 |
| Exogenous control 4 | 16 | 2.38 | 0.06 | 2.24 | 0.04 | 2.32 | 0.05 | 2.15 | 0.04 |
| Exogenous controls 5–48 | 43 | 2.49 | 0.20 | 2.23 | 0.05 | 2.47 | 0.22 | 2.15 | 0.05 |
| All controls spots | 107 | 2.43 | 0.15 | 2.23 | 0.05 | 2.39 | 0.17 | 2.15 | 0.04 |
| 635 nm | 532 nm | |
|---|---|---|
| # Apis cDNAs > background + 1 SD | 7308 | 7311 |
| # Apis cDNAs > background + 2 SD | 7300 | 7305 |
| # Apis cDNAs > control spots + 1 SD | 7029 (96%) | 7035 (96%) |
| # Apis cDNAs > control spots + 2 SD | 6647 (91%) | 6631 (90%) |
All inteneity values were log10 transformed. Feature and background readings are indicated for each cDNA spot (based on median pixel intensity) for 635 and 532 nm wavelengths. Average (Avg.) and standard deviation (SD) are indicated. Exogenous controls are described in Methods.