Table 11.

Homology Size (In Nucleotides) vs. Sensitivity for Four Search Criteria as Applied to Mouse/Human Comparisons Assuming 86% Nucleotide Identity and 89% Amino Acid Identity

	20	30	40	50	60	70	80	90	100	110	120	130
A	0.625	0.805	0.881	0.927	0.962	0.977	0.986	0.993	0.995	0.997	0.999	0.999
B	0.000	0.394	0.687	0.851	0.932	0.932	0.970	0.987	0.995	0.998	0.999	0.999
C	0.483	0.733	0.862	0.929	0.963	0.965	0.981	0.990	0.995	0.997	0.999	0.999
D	0.000	0.783	0.783	0.953	0.953	0.953	0.990	0.990	0.998	0.998	1.000	1.000

The median size of a human exon is 120 bases (International Human Genome Sequencing Consortium 2001). A, perfect 5 base amino acid match; B, two perfect 4-base amino acid matches within 100 amino acids and on diagonal; C, near-perfect 12 nucleotide match; D, near-perfect 8 amino acid match.