Figure 3.

Schematic representation of physical locations and orientations of the integrated transgene clusters and genomic mouse fragments, recovered as parts of mutant plasmids, on chromosomes 3 and 4. The red (proximal) and green (distal) arrowheads indicate the direction of the recovered mouse fragments, such that the base and tip of the arrowhead represent the breakpoint and the HindIII site, respectively (yellow downward arrow indicates direction unknown). The blue arrow indicates the location and orientation of the integrated transgene cluster (see also Fig. 1). The rounded physical distances from the integration site to the localized breakpoints in the mouse genome are shown. C indicates centromere; T, telomere.