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Chemical yield. A plot of the results of the stepwise yield of the NPPOC chemistry in the MAS system. Oligonucleotides with different numbers of bases were synthesized ranging from 1 to 12 bases. A biotin phosphoramidite was coupled to the end of all the oligonucleotides. Streptavidin-cy3 was added to visualize the relative amounts of free hydroxyls in each feature. The data represent the relative numbers after subtraction of background.
