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. 1998 Feb 3;95(3):1319–1324. doi: 10.1073/pnas.95.3.1319

Figure 1.

Figure 1

Estimation of mitochondrial contamination in preparations of thylakoid membranes. The TM and TP preparations of pea thylakoid membranes and mitochondrial membranes (Mito) were separated by SDS/PAGE and immunoblotted with antibodies specific for cytochrome oxidase subunit 1 (Cox1). The bottom line indicates NADH:FeCN activity corresponding to the amounts of protein loaded on the gel. A stromal lamellae (SL) fraction of thylakoid membranes, which is enriched in NADH:FeCN activity and Ndh proteins content (8, 10) is shown for comparison.