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(A) Alignment of codon 236–238 for the normal (R238) and mutant (R238X) FMO3 allele in cattle. This region was analyzed using pyrosequencing. The location of the sequencing primer and the six positions sequenced are indicated. (B) Pyrosequencing results from the three different genotypes at codon 238. The sequenced reverse strand has the sequence (A/G)AGTGA, in which the A/G polymorphism corresponds to the R238X nonsense mutation.
