Table 2.
Primer Sequences Used for PCR Amplification of the Bovine FMO3 Gene From Genomic DNA
| FMO3 region amplifieda | Forward (F) and reverse (R) primer sequences 5′–3′ |
|---|---|
| exon 3 | F: GACCATGCAGAAGAGGGCAG |
| R: CTTAAACTGTATGTATTTCAGGAGGTT | |
| exon 6 | F: CATCATCAGCTCCAGAAGTGG |
| R: TAAAGGCATCAAGCCATAGTT | |
| exon 7 | F: CAGAATCCTGAGGAAAGAGCC |
| R: ATTACTTGTGCTGCCCAGCG | |
| exon 8-9 | F: GATGAATGATATTAATGAGAAAATGG |
| R: CCGGTCCCACTGGGTCAG |
a
The numbering of exons is based on the described exon-intron organization for the human FMO3 gene (Dolphin et al. 1997).