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Southern blot analysis of clones modified by the pLD53.SC1 shuttle vector with the built-in resolution strategy. Co-integrates and resolved BAC DNA were digested with SpeI, separated by electrophoresis on a 1% agarose gel, and transferred to nylon membrane. The blots were analyzed using the “A Box” as probes. Controls used were wild-type BAC DNA (lane B) and shuttle vector (lane V).
