Figure 4.

Expression of a promoterless GUS construct fused to dspE in E. amylovora Ea273. Ea273 and Ea273dspE∷uidA (a merodiploid containing both a wild-type dspE and a truncated dspE fused to the uidA gene; solid bars) were grown in LB or Hrp MM, or inoculated to immature pear fruit. Ea273dspE∷uidAhrpL∷Tn5 (darkly shaded bar) and Ea273hrcV∷Tn5uidA (lightly shaded bar) were also grown in hrp MM. Values shown represent means of triplicate samples normalized for bacterial cell concentration. Standard deviations are represented by lines extending from each bar. The mean values for three samples of Ea273 in each assay were subtracted from, and standard deviations added to, the corresponding values obtained for the other strains.