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. 2002 Dec;12(12):1935–1942. doi: 10.1101/gr.547002

Figure 4.

Figure 4

Detection of 7028 C-T SNP in the COX1 gene with the DSNP assay. PCR fragments of 69, 534, and 952 bp comprising 7028 T or 7028 C variants, and pT-Adv plasmids with 69-bp fragment insertions were used in SNP typing with a T variant-specific signal probe. “DSN+”, reaction with DSN; “DSN−”, control samples, no enzyme. ΔFm/ΔFp was defined as in Table 1. (A) The multiwell PCR plate was photographed with an Olympus SZX12 fluorescent stereomicroscope equipped with a green filter. (B) PCR strips with DSNP results for 69-bp fragments were photographed with a MultiImage Light Cabinet (Alpha Innotech) under UV light.