Figure 1.

Targeted disruption of the m-numb gene. (A) Schematic drawings of the targeting vector, the wild-type, the floxed, and the mutant m-numb alleles. Exons 5 and 6 encode amino acids 68–139 of the m-Numb protein. All EcoRI (E) and HindIII (H) sites are shown, whereas only the relevant BamHI (B), XhoI (O), and XbaI (X) sites are shown. Open triangles represent PCR primers as described in Materials and Methods. (B) PCR analysis of the wild-type (+), floxed (flox), and mutant (Δ5,6) m-numb alleles.