Figure 4.

Graphs of protein spot densities represent the time course of heparin's effects on levels of proteins with the greatest change in expression, as detected in 2D gels and identified by MS, due to heparin instillation in rat lung. A. Calcium signaling pathway proteins indicated increases in ANXV, ANXVI, calcyclin, ARP3, and moesin (Msn) at 24 hours. While by 96 hours all were reduced from 24 hour levels, only calcyclin was reduced to below 0 hour control levels. B. Cytoskeletal domain proteins RhoGDI, septin, CapG, PIPTN, and HSP70 were increased at 24 hours. All decreased to control levels (0 hours) or less by 96 hours. C. Two immune response-related proteins, IGIP/PA28 and PSMA-1, were increased at 24 hours, and decreased by 96 hours. D. Two proteins relating to tumor suppression, DJ-1/PARK-7 and HABP-1, were increased at 24 hours and reduced by 96 hours, while Transgelin was reduced from control levels at 24 hours and remained low through 96 hours after heparin treatment. Note that Arp3 and Msn are found in the cytoskeletal domain as well as being grouped with the calcium signaling proteins but were graphed only once in A.