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. 2007 Jun 6;2(6):e496. doi: 10.1371/journal.pone.0000496

Figure 1. Images showing primary and secondary micro concavities at scanning electron and confocal microscopy.

Figure 1

(A) Primary micro concavity (arrow) of the PLGA surface at SEM. Cells can be completely contained within a primary concavity, due to its dimensions. (Calibration Bar = 10 µm); (B) SEM analysis of primary concavity dimensions (Calibration Bar = 10 µm); (C) SEM analysis of secondary concavity dimensions (Calibration Bar = 10 µm); (D) The interaction between the concave surface, showing primary (white arrow) and secondary (red arrows) micro-concavities at the confocal microscope (in green a cell within a concavity). The intimate adherence of a cell to the polymer surface and its nuclear polarity are clearly observable. The image was been obtained superimposing dark field with light field confocal microscopy (Calibration Bar = 10 µm); (E) Confocal image showing primary (outlined in red) and secondary (outlined in blue) micro-concavities and spider-shaped cellular elongations (Calibration Bar = 10 µm); (F) A gingival fibroblast not showing cellular alterations or nuclear polarity at the confocal microscope (Calibration Bar = 10 µm).