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. 2007 Jun 6;2(6):e496. doi: 10.1371/journal.pone.0000496

Figure 7. Immunofluorescence confirming the presence of a mineralized extra cellular matrix on concave texturing.

Figure 7

The panel shows positivity for Collagen I (A) FITC (green) (Calibration Bar = 10 µm), BAP [Bone Alkaline Phosphatase] (B) FITC (green) (Calibration Bar = 5 µm), OC [Osteocalcin] (C) PE (red) (Calibration Bar = 7 µm), ON [Osteonectin] (D) FITC(green) (Calibration Bar = 7 µm) and BSP [Bone Sialoprotein] (E) PE (red) (Calibration Bar = 3 µm). The same analysis confirming the presence of a mineralized extra cellular matrix on smooth texturing. The panel shows positivity for Collagen I (F) FITC (green) (Calibration Bar = 5 µm), BAP (G) FITC (green) (Calibration Bar = 7 µm), OC (H) PE (red) (Calibration Bar = 3 µm), ON (I) FITC (green) (Calibration Bar = 7 µm) and BSP (J) PE (red) (Calibration Bar = 3 µm). Nuclear staining is obtained with DAPI (blue).