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. Author manuscript; available in PMC: 2007 May 23.
Published in final edited form as: Biochemistry. 2004 Oct 26;43(42):13452–13458. doi: 10.1021/bi048892t

FIGURE 2.

FIGURE 2

(a) Gel mobility shift assay for Gq1 binding to the Htemp 50-mer DNA template. The DNA concentration was fixed at 1 nM, while the concentration of Gq1 protein added to the binding reaction was varied as shown above each lane. Binding was carried out in 100 mM K+ to promote G-quadruplex formation. (b) Equilibrium binding curve obtained by calculating the fraction of Htemp bound at varying Gq1 concentrations (ImageQuant software). The binding constant (Kd) was determined by fitting to the equation Ø = [P]/{Kd + [P]} (see the Materials and Methods).