Figure 1. SDS/PAGE profiles of a wheat CEC fraction and the isolated xylanase inhibitors.

A CEC fraction (lane 1) was obtained by fractionation of wheat wholemeal extract by CEC. Pure TAXI (lane 2), XIP (lane 3) and TLXI (lanes 4 and 5) were obtained in this order from the CEC fraction by affinity chromatography on B. subtilis and A. niger GH11 xylanase-affinity columns. TLXI was analysed by SDS/PAGE under non-reducing (lane 4) and reducing conditions (lane 5). The size of the molecular mass (MM) markers (lane 6) is indicated on the right-hand side in kDa. The gel was silver-stained.