Figure 4. Adrenaline activates AMPK and ACC phosphorylation in isolated fat cells.

Isolated fat cells from rats were treated with different doses of adrenaline for 10 min. Extracts were prepared and used in an in vitro AMPK activity assay (A and B) or Western blot analyses using anti-AMPK Thr¹⁷² and anti-ACC Ser⁷⁹ antibodies (C and D). Both AMPKα1 (A) and AMPKα2 (B) activities were significantly increased by adrenaline treatment. Phosphorylation of AMPK Thr¹⁷² (C) and ACC Ser⁷⁹ (D) were significantly increased with adrenaline treatment. Results are means±S.E.M.; n=3–6 per group. *P<0.05, **P<0.01 and ***P<0.001 compared with basal.