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mT2R5-, hT2R7-, hT2R14-, hT2R43- or hT2R47-expressing membranes were reconstituted with 1 μM transducin and 1 μM Gβ1γ1 in the presence of 100 μM of the indicated test tastant, and GTP[S] (GTPγS) binding assays performed. [35S]GTP[S] binding determinations in the absence of test ligand were subtracted from previously reported values.
