Table 2. EC50 values for IR, IGFR and IR–IGFR hybrids as measured by BRET assay.
BRET assays were performed as descirbed in the legends for Figures 4 and 5. EC50 values were determined using GraphPad Prism by curve-fitting with a one-site competition model. The values are means±range for two independent experiments each performed in duplicate.
| EC50 (nM) | |||
|---|---|---|---|
| Receptor | Tags | Insulin | IGF-I |
| IR-A | − | − | − |
| IR-B | IR–luc/IR–YFP | 0.1±0.1 | − |
| IR-A–IR-B | IR–luc/IR–GFP | 0.06±0.02 | − |
| IR-A–IGFR | IGFR–luc/IR–GFP | 60±10 | 4.0±0.5 |
| IR-B–IGFR | IGFR–luc/IR–YFP | 40±5 | 4.0±1.0 |
| IGFR | IGFR–luc/IGFR–YFP | 70±12 | 1.0±0.5 |