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. 2007 Apr 12;403(Pt 3):565–571. doi: 10.1042/BJ20061275

Figure 1. Purification and characterization of L7.8-g3p*.

Figure 1

(A) The L7.8-g3p* DNA fragment with a length of 318 bp was isolated from phage cDNA and amplified by PCR. Sizes (in bp) are indicated. (B) SDS/PAGE gel of L7.8-g3p* purified with metal-affinity chromatography. The molecular mass of L7.8-g3p* was approx. 10 kDa. Molecular masses (in kDa) are indicated. (C) Binding of L7.8-g3p* to the gp41 core. The binding of L7.8-g3p* (starting from 0.5 μM) to N36(L8)C34 that was immobilized on the sensorchip CM5 were analysed by SPR. M13-g3p* (0.5 μM) was used as a negative control. RU, response units.