Table 1.
Primer designation | Primer sequence | Location | Type of analysis | Ann. t, C° | MgCl2, mM | Product size, bp |
MSe1-F | tgctccaggctcttctcaggc | BIN1 promoter CGI, see Fig. 1 | MSe PCR | 61 | 1.5 | 255 |
MSe1-R | ggagaacgaaagtggagaagc | |||||
MSe2-F | ggggctccgggcgcgttctcc | 69 | 1.0 | 606 | ||
MSe2-R | actgcccatctctgccatcgc | |||||
MSp1-F | gggaaaggaaatttaatttttttt | MSp sequencing | 59 | 1.0 | 218 | |
MSp1-R | aataataaccctcaactctccaaac | |||||
MSp2-F | ggataataagttgttttttaaagggttatt | 58 | 1.0 | 212 | ||
MSp2-R | ctccacctaatctcctttcctaaat | |||||
BIN1-CA1F | cccgattttcattcaggtgtt | BIN1 intron 5 | LOH | 60 | 1.5 | ~210 |
BIN1-CA1R | agttccgagtttgatgccagc | |||||
BIN1-CA2F | cttctacaccttccacaatca | 3 kb distal to BIN1 | 60 | 1.5 | ~130 | |
BIN1-CA2R | attcctttcctgtccctttgg | |||||
BIN1-tccF | ccccaccccacaacctgaagt | BIN1 intron 1 | 60 | 1.5 | ~200 | |
BIN1-tccR | tccctgtgtttcctggctaag |