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. 2007 May 15;2:9. doi: 10.1186/1750-1326-2-9

Table 1.

Binding of apoE3 and apoE4 molecules to emulsion particles

apoE concentration (μg/ml) 0.1 1 10 30
Weight ratio of PC per apoE apoE3 496 ± 97 23 ± 6 7 ± 2 8 ± 3
apoE4 269 ± 85 16 ± 6 6 ± 1 7 ± 1

Lipid emulsions were prepared by the method described previously [36] using a high-pressure emulsifier. The emulsion used had a particle size of 34.7 ± 5.2 nm and a weight ratio of TO/PC was 1.63 ± 0.07. For the generation of apoE-EM complex, apoE at various concentrations, from 0.1 to 30 μg/ml, was incubated with EM at PC concentration of 5.0 μg/ml in a 5-ml solution for 1 hr at room temperature. The apoE-EM complex was isolated and assayed by the untracentrifugation method as previously reported [24, 37]. The weight ratio of apoE and PC were determined as described in the Experimental Procedures. Data are means ± S.E. of four samples.

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