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(A) Rat lungs treated with bleomycin was lysed and immunoblotted for HSP47 protein. Levels of HSP47 protein at 2 and 4 weeks were analyzed using Western blotting as described. HSP47 was up-regulated in the Bleo group compared to the Bleo+AS group. (B) HSP47 signal intensities were quantified using an image analyzer based on immunoblot density. HSP47 expression is shown as a percentage of the control. Data is expressed as mean ± SEM. * p < 0.05 vs. control group.
