Fig. 3.
Fluorescence of acrylodan-labeled actin and actin/actin binding factor complexes. (A) Fluorescence spectra of acrylodan-labeled Cys-374 in rabbit actin and actin/actin binding factor complexes as indicated. (B) Time course of fluorescence increase during acrylodan modification of Cys-203 in yeast actin (red) and in its binary complexes with cofilin (blue), DNaseI (gray), GS1 (black), and latrunculin (cyan). GS1 and profilin overlap, and the latter is not shown. (Inset) Plots of ln(F − Fmax) versus time, from which the rate constants are deduced. (C) Fluorescence spectra of the acrylodan-labeled Cys-203 yeast actin and actin/actin binding factor complexes. Color codes follow those from B. Spectra of actin, actin/GS1, and actin/profilin overlap, and the latter two are not shown.