Fig. 3.

GPRK2 function is required downstream of Hh. (A and B) Expression of Engrailed (En, red) in wing discs carrying clones of Gprki-expressing cells induced in hs-FLP; Ubx/abx Gal4-lacZ/UAS-Gprk2i (B) corresponds to the red channel, and the clones are labeled in green. The expression of En is lost in anterior cells. A′ and B′ show Z sections of the clones shown in A and B, respectively. (C) Expression of Engrailed (En, red) in wing discs carrying clones of Hh-expressing cells induced in hs-FLP; Ubx/abx Gal4-lacZ/UAS-hh larvae (<hh>, green). En is activated in anterior cells both within hh-expressing cells (green) and in the surrounding wild-type cells. Individual channels are shown for En (red, C″) and Hh-expressing cells (green, C″). (D–E) Expression of Engrailed (En, red) in wing discs carrying clones of cells expressing Hh and Gprk2i (<hh+Gprk2i>, green). En is activated in anterior cells surrounding the clones but not within the clone itself. Individual channels are shown below for En (D′ and E″) and (Hh+Gprk2i)-expressing cells (E′). E–E″ are higher magnifications of the clone shown in C.